rabbit anti αvβ6 antibody  (Bioss)

Journal: Frontiers in Oncology

Article Title: In vivo evaluation of integrin αvβ6-targeting peptide in NSCLC and brain metastasis

doi: 10.3389/fonc.2023.1070967

Figure Lengend Snippet: Expression of integrin αvβ6 and uptake of G2-SFLAP3-Cy5.5 in cell lines. (A) Immunofluorescence stain of integrin αvβ6 in A549 and H1703 cell lines. (B) Cell binding images of G2-SFLAP3-Cy5.5 in A549, H1703, and G2-SFLAP3-blocked A549. (C, D) The corresponding average fluorescence intensity of immunofluorescence and cell binding assay, respectively (n=3). Data were presented as mean± SD, ****p < 0.0001.

Article Snippet: The cells were incubated with rabbit anti-integrin αvβ6 antibody (1:100, Bioss, bs-1356R) at 37°.

Techniques: Expressing, Immunofluorescence, Staining, Binding Assay, Fluorescence, Cell Binding Assay

Journal: Frontiers in Microbiology

Article Title: The Pseudomonas aeruginosa Secreted Protein PA3611 Promotes Bronchial Epithelial Cell Epithelial-Mesenchymal Transition via Integrin αvβ6-Mediated TGF-β1-Induced p38/NF-κB Pathway Activation

doi: 10.3389/fmicb.2021.763749

Figure Lengend Snippet: PA3611 but not TGF-β1 stimulated avβ6 protein expression. 16HBE14o- and RTE cells were treated with different concentrations of PA3611 (1, 5, and 10 μg/mL) for different durations (12, 24, and 48 h), and αvβ6 expression was detected by Western blotting (A–H) . 16HBE14o- and RTE cells were treated with PBS, TGF-β1 (2 ng/mL), PA3611 (10 μg/mL), and PA3611 (10 μg/mL) + TGF-β1-neutralizing antibody (10 μg/mL) for 48 h, and αvβ6 expression was detected by immunofluorescence staining (I,J) and Western blot (K,L) . The results are representative of three independent experiments. The data are presented as the mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, vs. the control group; # P < 0.05, ## P < 0.01, ### P < 0.001, vs. the TGF-β1 group.

Article Snippet: Cells were then fixed with 10% formalin, permeabilized with acetone and stained with rabbit anti-α-SMA, -vimentin, - E -cadherin, -ZO-1 or -integrin αvβ6 antibodies followed by Alexa Fluor-488-goat anti-rabbit or Alexa Fluor-568-goat anti-rabbit secondary antibodies (Invitrogen).

Techniques: Expressing, Western Blot, Immunofluorescence, Staining

Journal: Frontiers in Microbiology

Article Title: The Pseudomonas aeruginosa Secreted Protein PA3611 Promotes Bronchial Epithelial Cell Epithelial-Mesenchymal Transition via Integrin αvβ6-Mediated TGF-β1-Induced p38/NF-κB Pathway Activation

doi: 10.3389/fmicb.2021.763749

Figure Lengend Snippet: PA3611 stimulated TGF-β1 expression and induced EMT, which could be inhibited by blocking integrin αvβ6. 16HBE14o- cells were pretreated with the integrin αvβ6-blocking antibody 10D5 (10 μM) or a specific inhibitor of TGF-β1-Smad2/3 signaling, SB431542 (10 μM), for 2 h prior to incubation with PA3611 (10 μg/mL) for 48 h. The TGF-β1 levels in cell culture supernatants were evaluated by ELISA (A) . EMT-related proteins (B–F) and signaling pathway proteins (G–I) were detected using Western blotting. The results are representative of three independent experiments, and the data are presented as the means ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, vs. the control group; # P < 0.05, ## P < 0.01, ### P < 0.001, vs. the PA3611 group.

Article Snippet: Cells were then fixed with 10% formalin, permeabilized with acetone and stained with rabbit anti-α-SMA, -vimentin, - E -cadherin, -ZO-1 or -integrin αvβ6 antibodies followed by Alexa Fluor-488-goat anti-rabbit or Alexa Fluor-568-goat anti-rabbit secondary antibodies (Invitrogen).

Techniques: Expressing, Blocking Assay, Incubation, Cell Culture, Enzyme-linked Immunosorbent Assay, Western Blot

Journal: Frontiers in Microbiology

Article Title: The Pseudomonas aeruginosa Secreted Protein PA3611 Promotes Bronchial Epithelial Cell Epithelial-Mesenchymal Transition via Integrin αvβ6-Mediated TGF-β1-Induced p38/NF-κB Pathway Activation

doi: 10.3389/fmicb.2021.763749

Figure Lengend Snippet: Effects of PA3611 on lung infection and EMT in vivo . Six- to eight-week-old male Wistar rats were intratracheally infected with the PAO1 or ΔPA3611 bacterial strain. The strains were coated with agarose and agarose, and PBS was used as a control. Lungs were collected at 2, 4, or 6 weeks post-infection. Overall gross views of lung tissues from the four groups at 2, 4, and 6 weeks post-infection are shown (A) . Bacterial load tests were performed in the above four groups at 2, 4, and 6 weeks post-infection (B) . HE staining (C) and immunohistochemical staining of E -cadherin, ZO-1, α-SMA, vimentin and integrin αvβ6 (D) were performed. The scale bar represents 100 μm. Morphometric analysis of EMT-related protein-positive areas and integrin αvβ6 was performed on immunohistochemically stained sections of lung tissues (E) . The lung injury results are representative of three independent experiments. The data are presented as the mean ± SD, NS, no significance, * P < 0.05, ** P < 0.01, *** P < 0.001, vs. the control group.

Article Snippet: Cells were then fixed with 10% formalin, permeabilized with acetone and stained with rabbit anti-α-SMA, -vimentin, - E -cadherin, -ZO-1 or -integrin αvβ6 antibodies followed by Alexa Fluor-488-goat anti-rabbit or Alexa Fluor-568-goat anti-rabbit secondary antibodies (Invitrogen).

Techniques: Infection, In Vivo, Staining, Immunohistochemical staining